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Djivan Prentout

@djivanp.bsky.social
97 followers 102 following 33 posts

Postdoc in evolutionary genomics, studying the mechanisms of meiotic recombination at Columbia University (Przeworski lab)

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Djivan Prentout @djivanp.bsky.social · 06/09/2024
14/ As expected in the absence of PRDM9 (tinyurl.com/2tjkar3j), recombination events overlap with CpG islands more often than expected by chance (with little or no effect of TSS).
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Djivan Prentout @djivanp.bsky.social · 06/09/2024
13/ In contrast, micro-chromosomes had significantly higher CO rates (4.21 cM/Mb), reflecting CO homeostasis. Both CO and NCO events were more uniformly distributed here than on macro-chromosomes.
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Djivan Prentout @djivanp.bsky.social · 06/09/2024
12/ Focusing on recombination, zebra finches show a sex-averaged CO rate of 1.05 cM/Mb on macro-chromosomes. COs are enriched near telomeres, while NCOs are more evenly spread. That too resembles the pattern in mammals.
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Djivan Prentout @djivanp.bsky.social · 06/09/2024
11/ Classifying mutations by developmental timing revealed a paternal germline mutation bias (1.7:1) in later stages of gametogenesis, but no sex difference in mutation rates during early development—similar to mammals (e.g., tinyurl.com/56zy44yh).
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Djivan Prentout @djivanp.bsky.social · 06/09/2024
10/ We estimate a sex-averaged mutation rate per bp per generation of 5.0 × 10⁻⁹ in zebra finches, which is comparable to other mammals and birds with similar generation times (tinyurl.com/w8wzkz4x).
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Djivan Prentout @djivanp.bsky.social · 06/09/2024
9/ To explore whether mutation and recombination parameters are similar in birds, we sequenced three-generation pedigrees of zebra finches, gathering information from 80 meiosis. We inferred 202 de novo mutations, 1,174 crossovers (COs), and 275 non-crossovers (NCOs).
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Djivan Prentout @djivanp.bsky.social · 06/09/2024
14/ As expected in the absence of PRDM9 (tinyurl.com/2tjkar3j), recombination events overlap with CpG islands more often than expected by chance (with little or no effect of TSS).
100
Djivan Prentout @djivanp.bsky.social · 06/09/2024
13/ In contrast, micro-chromosomes had significantly higher CO rates (4.21 cM/Mb), reflecting CO homeostasis. Both CO and NCO events were more uniformly distributed here than on macro-chromosomes.
100
Djivan Prentout @djivanp.bsky.social · 06/09/2024
12/ Focusing on recombination, zebra finches show a sex-averaged CO rate of 1.05 cM/Mb on macro-chromosomes. COs are enriched near telomeres, while NCOs are more evenly spread. That too resembles the pattern in mammals.
100
Djivan Prentout @djivanp.bsky.social · 06/09/2024
11/ Classifying mutations by developmental timing revealed a paternal germline mutation bias (1.7:1) in later stages of gametogenesis, but no sex difference in mutation rates during early development—similar to mammals (e.g., tinyurl.com/56zy44yh).
100
Djivan Prentout @djivanp.bsky.social · 06/09/2024
10/ We estimate a sex-averaged mutation rate per bp per generation of 5.0 × 10⁻⁹ in zebra finches, which is comparable to other mammals and birds with similar generation times (tinyurl.com/w8wzkz4x).
100
Djivan Prentout @djivanp.bsky.social · 06/09/2024
9/ To explore whether mutation and recombination parameters are similar in birds, we sequenced three-generation pedigrees of zebra finches, gathering information from 80 meiosis. We inferred 202 de novo mutations, 1,174 crossovers (COs), and 275 non-crossovers (NCOs).
110