These are nasal brushing (BMI1NHNE) cells. Grown in ALI culture then scraped off and put in a coverslip base 35mm dish for imaging.
We tried 40x1.15 Water and 63x1.4 Oil DIC lenses, both with 1.5x tubelens on a Ti2 stand. Kinetix camera cropped to 512x512 pixels to get the high frame rate at 12-bit.
