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Dr. Caleb Stoltzfus

@cstoltzfus.com
376 followers 2K following 26 posts

Optics | Microscopy | Physics | Image Analysis | Anything with lasers and data science

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Dr. Caleb Stoltzfus @cstoltzfus.com · 31/12/2025
When it stops raining for a minute Seattle is beautiful
Downtown Seattle at sunsetMount Rainier at sunset
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Dr. Caleb Stoltzfus @cstoltzfus.com · 21/03/2025
This workflow is very nice for organoids, where you want to take your time to pick and choose which organoids you re-image at sub-cellular resolution.
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Dr. Caleb Stoltzfus @cstoltzfus.com · 21/03/2025
The team published LUMI v2.0! The best new feature defines ROIs on low-res 3D scout scans! Scan your whole tissue at low resolution Define a bounding box using something like BigStitcher Import and scan the ROI Take your time finding and defining an ROI without worrying about photobleaching!
LUMI - light-sheet microscope control software main user interface. Both low resolution and high resolution camera feeds are simultaneously displayed on the right. Scan bounds, estimated scan time, laser control, and other control features are on the left of the screen.
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Dr. Caleb Stoltzfus @cstoltzfus.com · 20/12/2024
I hope I never get tired of how amazing cells look in 3D. Happy Holidays and Happy #FluorescenceFriday! Here are CD45+ cells in a skin biopsy. The segmented cells and nerves at the end of the video kind of look like strings of lights
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Dr. Caleb Stoltzfus @cstoltzfus.com · 06/12/2024
For #fluorescencefriday here is a mouse brain stained with Eosin and To-PRO-3 showing a segmented Glioblastoma in yellow. While this whole brain computational H&E staining is cool, I think I prefer TH staining for the dendrites. Imaged on Alpenglow Bioscience's 3Di #light-sheet #microscopy
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Dr. Caleb Stoltzfus @cstoltzfus.com · 06/12/2024
From Maple Leaf
Sunrise
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Dr. Caleb Stoltzfus @cstoltzfus.com · 27/11/2024
Very cool work! I really like the videos showing off the segmentation
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Dr. Caleb Stoltzfus @cstoltzfus.com · 22/11/2024
Here is a murine heart video we imaged with the Red-Horse lab for #FluorescenceFriday.
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Dr. Caleb Stoltzfus @cstoltzfus.com · 21/11/2024
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Dr. Caleb Stoltzfus @cstoltzfus.com · 21/11/2024
There are some really cool supplemental videos
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Dr. Caleb Stoltzfus @cstoltzfus.com · 17/11/2024
This is the best one I've found this fall
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Dr. Caleb Stoltzfus @cstoltzfus.com · 14/11/2024
Lets start this off with some 3D imaging of human placenta. Samples from Mira Moufarrej and the lab of Kristy Red Horse at Stanford. Imaged at Alpenglow Biosciences in Seattle by our amazing team of scientists and engineers.
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