Enhanced STAT3 activation combined with TGF-β inhibition reprograms human PSCs into cells specifying all early embryonic lineages. Upon 3D suspension culture, these cells self-organize within 6 days into post-implantation structures mimicking Carnegie stages 5–7 embryos, including gastrulation, with high morphological and molecular fidelity.

Enhanced STAT3 activation combined with TGF-β inhibition reprograms human PSCs into cells specifying all early embryonic lineages. Upon 3D suspension culture, these cells self-organize within 6 days i...

A small-molecule-only strategy enables reprogramming ESCs into embryo founder cells that give rise to a high-fidelity embryo model directly and efficiently. This embryo model replicates embryogenesis from initial cell fate specification through E8.5–E8.75-like organogenesis, capturing all key developmental features within a single system.