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Spine Lab at UIC

@boschlab.bsky.social

12 followers 9 following 10 posts

Synaptic plasticity and synaptophaties Lab, Dept Biomed Sc, UIC. Spines, waves, autism, photopharma, mapping

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Spine Lab at UIC @boschlab.bsky.social · 31/10/2025

Beware giving this site your unpublished data open.substack.com/pub/ubadah/p...

open.substack.com

Beware giving this site your unpublished data

Users noticed the website had fake testimonials and a domain name registered seemingly overnight.

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

In conclusion: TCP9 is a unique non-invasive tool for durable labeling, reversible photoswitching, and functional tracking of native receptors in brain tissue without genetic manipulation.

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

TCP9 photocontrols single neurons and single synapses in Xenopus tadpole brain in vivo

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

TCP9 photocontrols single neurons and synapses in ex vivo rat brain tissue, and allows tracking endogenous receptor physiology during long-term depression (LTD) in hippocampal neurons.

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

TCP9 enables photocontrolling endogenous GluRs in dissociated neurons at 1–10 Hz

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

TCP9 covalently targets AMPA and kainate receptors, maintaining their functionality and photoswitchability for extended periods (>8 h) after a single compound application.

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

TCP9 allows photoactivation of neuronal ensembles, individual neurons, and single synapses in ex vivo tissue and in intact brain in vivo, which is challenging using optogenetics and neurotransmitter uncaging.

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

We combine the photopharmacological effector TCP9 with neuronal activity sensors to demonstrate all-optical reversible control of endogenous GluRs across multiple spatiotemporal scales in rat brain tissue ex vivo and in Xenopus tadpole brains in vivo.

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

Since there was a lack of non-genetic methods to control the activity of endogenous proteins with pharmacological and spatiotemporal precision, we developed targeted covalent photoswitchable (TCP) compounds to control endogenous glutamate receptors (GluRs) using light.

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Spine Lab at UIC @boschlab.bsky.social · 30/09/2025

New paper by @miquelbosch.bsky.social, in Brain Stimulation: "Photoswitching endogenous glutamate receptors in neural ensembles and single synapses in vivo". A huge effort by Aida Garrido-Charles, Gorostiza Lab @ibecbarcelona.eu and great collaborators, www.sciencedirect.com/science/arti...

sciencedirect.com

Photoswitching endogenous glutamate receptors in neural ensembles and single synapses in vivo

To interrogate animal physiology in vivo, there is a lack of non-genetic methods to control the activity of endogenous proteins with pharmacological a…

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