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Biswajit Pradhan

@bislightyear.bsky.social
181 followers 161 following 22 posts

Postdoc at MPI Biophysics. DNA, SMC, Chromosome Organization, Nanoscience, Single-Molecule Biophysics. www.biswajit.de

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Biswajit Pradhan @bislightyear.bsky.social · 02/12/2024
On a surface-anchored plasmid, Wadjet extrudes a DNA loop until it reaches the anchor point. The complex then stalls, ultimately cleaving the plasmid at the anchor.
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Biswajit Pradhan @bislightyear.bsky.social · 02/12/2024
Here is a direct visualization of DNA loop extrusion under buffer flow. We found that Wadjet extrudes DNA as a dimer, and LE is inhibited when dimerization is disrupted.
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Biswajit Pradhan @bislightyear.bsky.social · 02/12/2024
Happy to see our research on SMC Wadjet published in Molecular Cell. We showed DNA loop extrusion (LE) by a bacterial SMC, confirming LE is conserved across all life forms. Wadjet's LE activity is crucial for recognizing and restricting foreign plasmids, serving as a defense mechanism. 🧬🔬🧪
Graphical abstract showing the loop extrusion mechanism of the Wadjet complex, which selectively cleaves circular DNA while leaving linear DNA unaffected.
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