Luis Miguel Cerron-Alvan @biolm.bsky.social · 16/04/2026Preprint 👇 (4/4) www.biorxiv.org/content/10.6...biorxiv.org 030
Luis Miguel Cerron-Alvan @biolm.bsky.social · 16/04/2026In the E5.5 epiblast, WT1 expression and splice composition align with lineage-biased transcriptional states. This suggests lineage-associated programmes begin to diversify before gastrulation, with WT1 acting as an isoform-tuned regulator of early lineage bias. (3/4) 120
Luis Miguel Cerron-Alvan @biolm.bsky.social · 16/04/2026WT1 biases transcriptional programmes and is sufficient to drive post-implantation identity. Alternative splicing produces WT1 isoforms with distinct effects: → one biases anterior / neural programmes → another biases posterior / mesodermal programmes (2/4) 100
Luis Miguel Cerron-Alvan @biolm.bsky.social · 16/04/2026When do lineage biases first emerge in the epiblast? They are detectable before gastrulation, but how this early asymmetry is established has remained unclear. A CRISPRa screen identifies WT1 as an unexpectedly early regulator of formative pluripotency. (1/4) 171
