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Bas Bargmann

@bas-bargmann.bsky.social
195 followers 229 following 45 posts

Associate Professor Virginia Tech School of Plant and Environmental Sciences

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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
Lastly, we were also able to visualize the development of transcriptional asymmetry in a growing microcolony. Using a dual auxin- and cytokinin-signaling reporter-gene construct, it was seen that the reporter expression starts to diverge into distinct domains in more mature microcolonies. (10/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
We managed to capture the donut-shaped structure of microtubules surrounding a phragmoplast forming between two recently separated nuclei. Thanks to Emry Ng for the image processing! (9/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
We also used a Lattice Lightsheet microscope to perform high-temporal resolution live imaging of cultured cells. This set up allowed us to catch a dividing cell in the act. Our favorite is an example showing the microtubule dynamics during the first division of a regenerating protoplast. (8/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
We also showcased the use of additional cellular markers, for the Golgi and microtubules. Confocal Z-stacks of developing microcolonies demonstrate the intricate details of intracellular organization of cellular compartments and components during the early stages of regenerative development. (7/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
Furthermore, the inclusion of fiducial fluorescent beads embedded within the alginate discs allowed us to find and track the same cells over time. (6/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
To visualize the development of protoplast regeneration microscopically with enhanced detail and resolution, we incorporate fluorescent cellular markers for the plasma membrane and the nucleus. To better distinguish cell-division events, which can be challenging with brightfield microscopy. (5/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
Part of the novelty of this method is the embedding of protoplasts in alginate discs that fit into the wells of a 24-well plate. This format “enables high throughput experimental design: a single isolation yields more than 24 discs, each potentially representing an independent treatment.” (4/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
This work was initiated over seven years ago, when Kelsey first started to develop a protoplast regeneration system using Arabidopsis. There were several methods already published, but we struggled to make them work in our hands. We hope that the method used here will be more transferrable. (3/n)
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
It is titled “A protoplast-based method to visualize early cell biological events in plant cellular reprogramming and regeneration” and that basically says it all. Nonetheless, I will take this opportunity to walk you through the main points. We also made the cover! #FluorescenceFriday (2/n)
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Bas Bargmann @bas-bargmann.bsky.social · 11/08/2026
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Bas Bargmann @bas-bargmann.bsky.social · 01/07/2026
Such fun hosting @julianverdonk.eurosky.social for a mini sabatical (Virginia Tech Translational Plant Sciences Center Global Distinction Fellow) in the lab! Been messing with science together since the 90's 🤓
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Bas Bargmann @bas-bargmann.bsky.social · 29/05/2026
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Bas Bargmann @bas-bargmann.bsky.social · 29/05/2026
The workshop was a great success 🤓 So much so that one of our organizers was elected into the Royal Society! 🤴 Thanks to all participants!!
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Bas Bargmann @bas-bargmann.bsky.social · 27/05/2026
Great first day of our NSF PGRP sponsored maize transformation workshop at Rutgers with Andrea Gallavotti, Margaret Young @revealplant.bsky.social, Dave Jackson, and special guest Kan Wang! Wonderful group of participants getting their hands dirty in the lab 🤓
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Bas Bargmann @bas-bargmann.bsky.social · 10/05/2026
Great to see such a wonderful turnout!
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Bas Bargmann @bas-bargmann.bsky.social · 14/09/2025
This should be fun! 🤓 Join us May 10-13 in Barcelona!! www.ptcge2026.com
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Bas Bargmann @bas-bargmann.bsky.social · 11/05/2025
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Bas Bargmann @bas-bargmann.bsky.social · 08/05/2025
Congratulations to Dr. Joseph Taylor for successfully defending his dissertation, “Decoding the Transcriptional Specificity of Auxin Signaling: A Synthetic Biology Approach" earlier today!
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Bas Bargmann @bas-bargmann.bsky.social · 06/05/2025
A pleasure to host Dave Jackson for a wonderful SPES seminar today!
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Bas Bargmann @bas-bargmann.bsky.social · 21/12/2024
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Bas Bargmann @bas-bargmann.bsky.social · 21/12/2024
Got to dress like a wizard yesterday 🤓 Congrats again to Dr. Reed!!
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Bas Bargmann @bas-bargmann.bsky.social · 18/11/2024
Conditional expression of a gain-of-function Aux/IAA 🤓
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