iiinova — where your digital self keeps evolving

BaDoi Phan @badoiphan.bsky.social · 25/05/2025

This weekend I walked to at graduation to end my 8 years in Pittsburgh. This was supported by mentors and friends including Andreas Pfenning and Ruby Holland. To top it off, a major PhD dissertation project with @wrstauffer.bsky.social is now published in Neuron! bit.ly/4ktg1v1

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BaDoi Phan @badoiphan.bsky.social · 21/03/2025

I'm excited to be matched to at @stanfordanes.bsky.social, an excellent program, and future home for me to continue my career as a physician scientist caring for the people from where I grew up. 🧑🏻‍⚕️👨🏽‍🔬 #doubledocs

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Darby Saxbe @darbysaxbe.bsky.social · 04/02/2025

🚨BREAKING. From a program officer at the National Science Foundation, a list of keywords that can cause a grant to be pulled. I will be sharing screenshots of these keywords along with a decision tree. Please share widely. This is a crisis for academic freedom & science.

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BaDoi Phan @badoiphan.bsky.social · 03/12/2024

I'm teaching single-cell data analysis in #Rstats this Spring 2025 @FAESatNIH_EDU ! 📊 Two-course series: Updated Seurat v5 tutorials + multi-modal analysis + spatial transcriptomics content Real datasets & self-directed projects Register @ tinyurl.com/FAESscBioS2025 #bioinformatics #scRNA

tinyurl.com

BUND 023 2-COURSE BUNDLE - Foundations for Practical Single Cell RNA-seq Analyses and Advanced Topics in Single Cell Analyses

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🌟 15/15 Impact: These tools enable new experiments in primate cognition & potential therapies. Grateful to both labs & collaborators for support! DM for questions! /end

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🧩 14/15 Biggest achievement: Built pipeline to identify & validate cell-specific tools in primates. Years of work and a huge team effort but worth it! 😮‍💨

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🔮 13/15 What's next: - Studying cognitive circuits - Developing targeted therapies - Understanding disorders Just getting started!

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🎓 12/15 Proud to share resources, contact @wrstauffer for details: - Primate DLPFC cell atlas🧬 - Our ML models💻 - Validated viral toolbox 🧰 🦠 All available to accelerate research! #OpenScience

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

⚠️ 11/15 We're transparent about limitations: - Some baseline non-specific expression - Promoter needs optimization - Viral crosstalk issues in multiplexing More work ahead!

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🐭 10/15 Surprising discovery: Our primate enhancers worked in mouse cortex too! Evolution conserved these regulatory elements 🧬 #evolution

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🔍 9/15 Our validation pipeline: - Multiplexed screening first - Single injections to confirm - Functional testing Rigorous approach pays off!

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🧪 8/15 Key finding: Getting specificity right depends on: - Viral titer (concentration matters!) - Capsid choice - Injection parameters Details matter!

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

⚡️ 7/15 Most exciting part: Used RMacL3-01 to express ChR2. Could control L2/3 neurons with light! First time achieving this specificity in primate DLPFC 🔦 #optogenetics

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🔋 6/15 Best performers: - Our RMacL3-01: Targets L2/3 projection neurons (77% specific!) - RMacL5ET-01: Hits L5 extra long-range projection neurons Proud of these results! 🎯

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🎯 5/15 Our viral toolbox: Created AAVs with these enhancers. Like GPS-guided delivery trucks for genes! Tested different viral capsids (PHP.eB, 2YF, X1.1) to optimize delivery 🚛

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🤖 4/15 The cool part: We developed ML models (SNAIL) to scan thousands of DNA regions & predict enhancers. Turned a needle-in-haystack problem into a targeted search! #MachineLearning

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🧬 3/15 Our approach: We used snRNA-seq & ATAC-seq from rhesus DLPFC. Found 11 excitatory & 10 inhibitory neuron types conserved in humans🧑🏽‍🦲and rodents🐭! Each with unique molecular fingerprints 🎯 #genomics

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🔬 2/15 The challenge: How do we study specific neuron types in primate brains? Prior to our work, we lacked tools to target DLPFC neurons, especially in a cell-type specific way #Neuroscience

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BaDoi Phan @badoiphan.bsky.social · 02/12/2024

🧠 1/15 First bsky post and preprint alert! Part 4 of my thesis work is now online🎓🕺📜! We created tools to study specific neuron types in primate prefrontal cortex to be able to study complex thinking! Let's break down this work from the Stauffer (Pitt 🐵) and Pfenning (CMU🧬) labs🧵.

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Guido Meijer @guidomeijer.com · 11/11/2024

I made a systems neuroscience starter pack, focused on experimentalists who study the brain at the level of populations of neurons! Also want to be on the list? Reply or DM me a picture of your ephys rig or 2p setup and I'll add you 🤭 go.bsky.app/UiqX7ry

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Jayme McReynolds @jaymemcreynolds.bsky.social · 10/11/2024

I made a starter pack for neuroscience folks but I just saw that it’s capped at 150 people. Sorry if I missed anyone! Maybe #neuroscience folks should start making subfield starter packs? 🧪 go.bsky.app/Q69XurQ

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Talia Lerner @talialerner.bsky.social · 10/11/2024

A starter pack! Some of my favorite women in neuroscience (sorry if I forgot you, I will add!) go.bsky.app/SCFmKoC

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BaDoi Phan @badoiphan.bsky.social · 12/11/2024

As an #R user both in #Rstudio and #Terminal, I have been known to change my IDE color panel to look like the #Matrix 😎💻💊

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BaDoi Phan @badoiphan.bsky.social · 11/11/2024

I have money on those being subtypes the medial shell NAcc islands of NUDAP MSNs 😉. #addiction #neurobiology

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Keri Martinowich @martinowk.bsky.social · 11/11/2024

Doing a #MicroscopyMonday because I’m just so excited about these images of D1R sub -populations in nucleus accumbens of human brain 🧠🔬🤩

Microscopy image of human brain with fluorescently labeled cell markers

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Mary Kay Lobo @marykaylobo.bsky.social · 11/11/2024

Hi Everyone 👋 Been slow to migrate over here but here I am! Neuroscientist studying addiction stress, circuits and molecules across the lifespan. Prof and Vice Chair of Neurobio and Assoc Director of the Kahlert Institute for Addiction Medicine.

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