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Antonio Serrano @antonioserrano.bsky.social · 08/12/2024

PhD position avaiable at the CBGP with Clara Sánchez in Madrid, Spain. Share to anyone interested please! Open to international and national students.

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Antonio Serrano @antonioserrano.bsky.social · 19/11/2024

In summary, our results reveal a substantial fluidization of the cytoplasm during morphogenesis, a key process for fungal virulence, via a reduction in ribosome concentration and that importantly inhibition of ribosome biogenesis triggers filamentous growth (8/11)

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Antonio Serrano @antonioserrano.bsky.social · 19/11/2024

Depletion of a protein critical for ribosome biogenesis resulted in a reduction of rRNA levels and an increase in GEM Deff. Strikingly this also resulted in the induction of the yeast-to-filament transition (both morphologically and of hyphal specific genes) w/o serum. (7/11)

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Antonio Serrano @antonioserrano.bsky.social · 19/11/2024

Mass spectrometry revealed that ribosomal protein levels drop during filamentation. To directly visualize this, we carried out Cryo-EM+2D template matching and observed that filamentous cells have a lower ribosome concentration, which was proportional to their length. (6/11)

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Antonio Serrano @antonioserrano.bsky.social · 19/11/2024

🧮 Mathematical modeling (both theoretical predictive equations and simulations) recapitulate our observations, that is the ~2-fold increase in Deff as cell volume increases. This supports our initial hypothesis: there is a substantial dilution of a cytoplasmic crowder. (5/11)

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Antonio Serrano @antonioserrano.bsky.social · 19/11/2024

We found GEM Deff increases 2x during filamentation, indicating that the cytoplasm becomes more fluid and likely less crowded. Fluidity correlates very nicely with filament length/volume, suggesting a substantial dilution of molecular crowders e.g. ribosomes. (4/11)

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Antonio Serrano @antonioserrano.bsky.social · 19/11/2024

The cytoplasm is highly crowded, and this affects a range of biological functions. We tracked how crowded the cytoplasm is during morphogenesis by using genetically encoded GEMs. Lower GEM effective diffusion (Deff) = higher crowding. This is the cytoplasm at 30 fps 🎥 (3/11)

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Antonio Serrano @antonioserrano.bsky.social · 19/11/2024

Using a passive fluorescent microrheological probe (GEMs), we reveal how cytoplasmic fluidity changes during yeast-to-filament morphogenesis, a key process for fungal virulence. (2/11)

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Antonio Serrano @antonioserrano.bsky.social · 16/11/2024

Enjoying the beauty of fungi during our last lab retreat in the mountains of Madrid! #Nature #Fungi

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Antonio Serrano @antonioserrano.bsky.social · 23/10/2023

PhD position in cell biology to work at the CBGP in Madrid, Spain. Project: Dynamic Cell Wall remodeling during plant-microbe interaction. To apply: clara.sanchez@csic.es (subject: CWPM-PhD). #PhDposition #sciencejobs. Share please!

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