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Aline Grata

@alinegrata.bsky.social
98 followers 215 following 20 posts

PhD student working on epithelial cell extrusion in Romain Levayer’s Lab | Social media for Piplettes Magazine ✍🏻 📍Pasteur Institute

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Aline Grata @alinegrata.bsky.social · 21/05/2025
We observed that AK7 depletion leads to defects in centriole docking, with centrioles remaining sub-apical and therefore failing to nucleate motile cilia.
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Aline Grata @alinegrata.bsky.social · 21/05/2025
The mechanism? Centrioles! Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
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Aline Grata @alinegrata.bsky.social · 21/05/2025
We also performed SEM imaging and observed that ciliary structures are impaired when AK7 is lacking, with reductions in both cilia number and length.
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Aline Grata @alinegrata.bsky.social · 21/05/2025
Loss of AK7 was related to PCD-like phenotypes, with decreased fluid flow and reduced beat frequency of MCCs. One of the challenges was to find a way to visualize live ciliary beating with a good enough spatiotemporal resolution (tipically cilia are around 15μm long with beating frequency of 15Hz).
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Aline Grata @alinegrata.bsky.social · 21/05/2025
We observed that AK7 depletion leads to defects in centriole docking, with centrioles remaining sub-apical and therefore failing to nucleate motile cilia.
100
Aline Grata @alinegrata.bsky.social · 21/05/2025
The mechanism? Centrioles! Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
100
Aline Grata @alinegrata.bsky.social · 21/05/2025
We also performed SEM imaging and observed that ciliary structures are impaired when AK7 is lacking, with reductions in both cilia number and length.
100
Aline Grata @alinegrata.bsky.social · 21/05/2025
Loss of AK7 was related to PCD-like phenotypes, with decreased fluid flow and reduced beat frequency of MCCs. One of the challenges was to find a way to visualize live ciliary beating with a good enough spatiotemporal resolution (tipically cilia are around 15μm long with beating frequency of 15Hz).
100
Aline Grata @alinegrata.bsky.social · 25/03/2025
Just wrapped up an amazing 2-week Principles and Applications of Fluorescent Microscopy #PAFM course @pasteur.fr! Built a microscope, designed a confocal, attended great talks, and got hands-on with the latest tech🔬💡 Great mix of theory and practice. 10/10, highly recommend!🤩 #microscopy #biology
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