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Alberto Macho

@albertopmacho.bsky.social

169 followers 138 following 13 posts

Scientist studying Plant-Pathogen Interactions (Molecular Biology - Biochemistry - Genetics). Scuba diving. Hiking. Football. Photography

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Alberto Macho @albertopmacho.bsky.social · 02/09/2025

These phosphorylation sites seem to be important for RipBM contribution to virulence in tomato, reflecting an effector strategy to promote its own stability and contribute to bacterial virulence

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Alberto Macho @albertopmacho.bsky.social · 26/07/2025

This reminds me the treatments with the flg22G/A mutant peptide we did in Sun et al, Science, 2013 (doi.org/10.1126/scie...). That mutation abolishes BAK1 interaction and activation, significantly reduces ROS, but does not affect MAPK activation

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Alberto Macho @albertopmacho.bsky.social · 26/07/2025

Long story short, it seems that AtFLS2, when expressed in N. benthamiana, associates with NbSERKs and mediate a “partial” complex activation that leads to some downstream responses (MAPK, gene expression), but not others (ROS). Eventually, this enhances disease resistance

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Alberto Macho @albertopmacho.bsky.social · 26/07/2025

We did not see any gain of flg22Rso-triggered ROS burst, but… there was MAPK activation. Surprisingly, what was supposed to be a “negative control”, even WT AtFLS2 expressed in N. benthamiana would confer MAPK activation upon flg22Rso treatment

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