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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(13/13) This work left us with another thought-provoking observation: the way these activation energies correlate is *not* evocative of an aboslute upper limit to the adaptation of microbial growth to high temperature... If it exists, such an absolute limit has to be caused by some other process!

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(12/13) the observed correlation between the maximal and optimal growth temperature which seems to hold universally in microbes. Such a pattern is expected if activation energies of both constructive and destructive processes increase with growth temperature while metabolic scaling does not.

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(11/13) This scaling remains relatively constant over different temperatures, signaling that it may not be as important a determinant of maximal growth temperature as activation energies. Together with the correlation found among activation energies, this sheds light on a thought provoking pattern:

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(10/13) the fraction of cellular molecules which participate to constructive processes (e.g. metabolic machinery) and those which incur maintenance costs (all of them)! Treating cell growth as the sum of two single-rate functions is fine enough as long as we account for this scaling.

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(9/13) Our analysis shows that such is likely not the case, i.e. that there is no analogy for enthalpy-entropy compensation at the scale of the entire cell (while it is still a thing at the molecular scale). Instead, this artifact disappears when the model accounts for scaling differences between

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(8/13) Likewise, a phenomenon called the 'enthalpy-entropy' compensation was thought to apply at the cell scale within the family of models that we study, explaining some observed correlations within parameters of the original formulation of the model by Hinshelwood in 1946.

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(7/13) it could also be caused by a trade-off directly at the cellular level e.g. whereby adaptation at high temperature is mediated by allocation of metabolic power to synthesis of stress-response proteins in detriment of synthesis of other functional proteins.

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(6/13) Our model being a coarse-grained description of cellular processes, and not explicitly of enzymatic rates, our findings cannot confirm the existence and relevance of this molecular-scale tradeoff. While such a molecular trade-off could cause this pattern at the cell level,

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(5/13) -surprisingly, the rate of 'constructive' processes is also inhibited in high temperature growing archaea. This could be the manifestation of a longstanding hypothesis: the enzymatic activity-stability tradeoff, which states that increasing enzyme stability decreases activity.

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(4/13) Linking empirical optimal and maximal growth temperatures to parameter values in Archaea showed that -organisms growing at higher temperatures have more inhibited rates for destructive processes, which makes perfect sense in the context of adaptation to high temperatures

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(3/13) We created a basic version of this family of models, suitable for parameter inference using data where the growth rate of an organism is reported at various temperatures. This allowed us to turn a database of measured growth rates into one of estimated parameter values.

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(2/13) Often, microbial growth is represented as the sum of a positive ("constructive") and negative ("destructive") temperature-dependent rate, reproducing the typical asymmetrical shape of microbial thermal growth curves. How does adaptation change the parameters defining these terms?

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Antonin Affholder @aaffholder.bsky.social · 18/11/2025

(1/13) Hey, BlueSky. I just got some new research published! "Activation energies of both constructive and destructive cellular biochemistry determine maximum growth temperature in archaea". What microbial growth parameters change with growth temperature? rdcu.be/eM8bv.

rdcu.be

Activation energies of both constructive and destructive cellular biochemistry determine maximum growth temperature in archaea

Communications Biology - Using Bayesian inversion on microbial growth curves, the difference between activation energies of molecular destructive and constructive processes is identified as the...

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Reposted by Antonin Affholder

Communications Biology @commsbio.nature.com · 04/11/2025

Using Bayesian inversion on microbial growth curves, the difference between activation energies of molecular destructive and constructive processes is identified as the main driver of variations in the maximum growth temperature of archaeal cells. @aaffholder.bsky.social go.sn.pub/c511xp

go.sn.pub

Activation energies of both constructive and destructive cellular biochemistry determine maximum growth temperature in archaea - Communications Biology

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Antonin Affholder @aaffholder.bsky.social · 04/09/2025

Tonight I'll be on SETI Live, talking about our recent work about the habitability of Saturn's moon Titan ! Tune in at youtu.be/a8tgpFdHgWo

youtu.be

Life in Titan’s Ocean? The Microscopic Possibility of Biomass on Saturn's Moon

YouTube video by SETI Institute

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Reposted by Antonin Affholder

Centre for Origin and Prevalence of Life (COPL) at ETH Zurich @copl-eth.bsky.social · 04/04/2025

We are delighted to announce the 2025 cohort of NOMIS-ETH postdoctoral fellows. Thomas Drant and @aaffholder.bsky.social are joining the COPL, thanks to the generous support of the NOMIS Foundation. copl.ethz.ch/news/COPL-ne... @ethzurich.bsky.social @eth-eaps.bsky.social @usyseth.bsky.social

copl.ethz.ch

2025 Cohort of the NOMIS-ETH Fellows

The Centre for Origin and Prevalence of Life announces the 2025 cohort of NOMIS-ETH Fellows.

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Antonin Affholder @aaffholder.bsky.social · 13/02/2025

Future directions include completing this coupled model with early-Earth type ecosystems in order to look at potential biosignatures and their detectability 😉. Will take time to do it right! S Mazevet, D Apai @danielapai.bsky.social , B Sauterey, R Ferriere @regisferriere.bsky.social

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Antonin Affholder @aaffholder.bsky.social · 13/02/2025

Taking a Bayes factor approach, we estimate that sampling ~25 exoplanets should allow us to answer this question. Target yields for near-future telescope designs are spot on to learn about exoplanet habitability 🎯.

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Antonin Affholder @aaffholder.bsky.social · 13/02/2025

OK, but will future telescopes observing Earth-sized exoplanets in the HZ be able to determine which of our two scenarios is most common? How likely detection of atmospheric CO2 is differs between our scenarios.

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Antonin Affholder @aaffholder.bsky.social · 13/02/2025

Some amount of CO2 regulation occurs in planets in our SL scenario for carbon cycling... provided that they are relatively young (<2 billion yrs). SL is not as good for sustained habitability, but many terrestrial HZ planets could be habitable, even if in the SL regime!

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Antonin Affholder @aaffholder.bsky.social · 13/02/2025

Lo and behold! The distributions of atmospheric CO2 as a function of orbit/luminosity are qualitatively different depending on the carbon cycle scenario (left: Earth-like; right:SL).

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Antonin Affholder @aaffholder.bsky.social · 13/02/2025

To tackle this question, we tried to set expectations for the atmospheric composition of exoplanets under two scenarios of carbon cycling: Earth-like and 'stagnant-lid' (SL) where atmospheric control of CO2 is weaker. Writing and running this model has been my toughest challenge so far.

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Antonin Affholder @aaffholder.bsky.social · 13/02/2025

We just got some new research published last week about exoplanet habitability : doi.org/10.3847/1538-3881/ada384 Earth's carbon cycle sustained by plate tectonics is unique in the solar system. Are terrestrial exoplanets in the HZ like the Earth? Or like Mars and Venus ?

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