Lucy Crouch @lucyicm.bsky.social · 18/07/2025Here we describe the first structure of a PNGaseA superfamily member. This structure has the canonical PNGase two β-sandwich catalytic module (pink/gold), but also a large unique β-sheet cradle (silver). 100
Lucy Crouch @lucyicm.bsky.social · 18/07/2025Biochemical characterisation revealed that this enzyme has a preference for N-glycans attached to peptides rather than denatured or native proteins, which suggests that microbes with these enzymes target these types of glycoproteins for use as a nutrient source. 100
Lucy Crouch @lucyicm.bsky.social · 18/07/2025The structure reveals a C-shaped pocket that likely accommodates peptides and modelling of peptides into this pocket supports this hypothesis. 100
