1/3 Metacells boost power in single-cell RNA-seq & multiome analysis. But without checking homogeneity, they risk forming dubious metacells that bias discoveries.
We introduce mcRigor: a statistical safeguard for rigorous metacell analysis.
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nature.com
mcRigor: a statistical method to enhance the rigor of metacell partitioning in single-cell data analysis - Nature Communications
Aggregating similar single cells into metacells is a common heuristic for sparse data, but risks mixing dissimilar cells. Here, authors present mcRigor, which detects and filters heterogeneous metacells, optimizes metacell partitioning, and improves reliability in single-cell omics studies.