Ali Haidar @ahaidar.bsky.social · 31/08/2026RNA-seq of mechanically isolated Zbs: 960 enriched mRNAs, encoding the very organelles inside it, and Mass spec finds 585 Zb proteins: ribosomes, and the PrLD helicases DDX3X and DDX5. 100
Ali Haidar @ahaidar.bsky.social · 31/08/2026OPP click chemistry and a fluorescent puromycin conjugate both show nascent peptides concentrating in the Zb, plus phospho-mTOR enrichment. in addition, quantitative phase imaging shows cluster-stage oocytes hold protein density constant as they grow. 110
Ali Haidar @ahaidar.bsky.social · 31/08/2026Supinoxin treatment (DDX5 inhibitor) dissolves the Zb in 2–4 h and flattens follicle and oocyte growth in live ovarian slices over 20 h, while RK-33 (DDX3X inhibitor) does neither. 010
