Mikael Marttinen @mmarttinen.bsky.social · 26/05/2025Lastly, SUM-seq is an ideal match for arrayed screens. We show this in a CRISPRi and CRISPRa, modulating key lineage TFs (GATA2/SOX17/NR4A2) in hiPSCs over a time course (0,4,12, and 18 DIV) of spontaneous differentiation (7/) 110
Mikael Marttinen @mmarttinen.bsky.social · 26/05/2025Constructing temporal GRNs revealed keys TFs at different stages of development and how perturbation of key lineage TFs shift developmental trajectories of cells (8/) 120
Mikael Marttinen @mmarttinen.bsky.social · 26/05/2025This was a long, fun project, which took the effort of many talented people (Sara Lobato, Umut Yildiz, @anniquec.bsky.social et al.) from the Zaugg and Noh labs! @embl.org @unibas.ch A detailed protocol is in the works for release. For now, check out the paper! 021
