choudharylabcpr.bsky.social @choudharylabcpr.bsky.social · 18/11/2025CTCF, however behaves differently. Acute CTCF loss also alters hundreds of genes, but the pattern is mixed (both up and down) and only some require CBP/p300 dependent enhancers. 100
choudharylabcpr.bsky.social @choudharylabcpr.bsky.social · 18/11/2025By endogenously mutating CTCF to disable cohesin anchoring, we show that promoter-proximal CTCF activates genes independently of enhancers and looping. Instead CTCF acts as an orientation-dependent transcription activator, primarily regulating essential housekeeping genes. 110
choudharylabcpr.bsky.social @choudharylabcpr.bsky.social · 18/11/2025The CTCF paralog CTCFL (BORIS) cannot anchor cohesin loops but can function as transcription activator to substitute for CTCF at promoter-regulated targets. This means it can disrupt 3D genome organization, but without hampering expression of CTCF-dependent essential genes. 100
