choudharylabcpr.bsky.social @choudharylabcpr.bsky.social · 09/12/2024Among diverse types of posited enhancers, cohesin seems to uniquely facilitate enhancer-mediated gene regulation through p300/CBP-dependent enhancers. 100
choudharylabcpr.bsky.social @choudharylabcpr.bsky.social · 09/12/2024Using a cohesin-loop-deficient CTCF mutant, we reveal that CTCF is a position- and orientation-dependent transcription activator at promoters. It directly activates hundreds of essential housekeeping genes—independently of cohesin and enhancers—even in plasmid reporter assays. 100
choudharylabcpr.bsky.social @choudharylabcpr.bsky.social · 09/12/2024The CTCF-like protein BORIS is also unable to anchor cohesin. Re-analysis of published data with this new perspective show, that unlike CTCF, BORIS has no impact on enhancer-regulated genes. In contrast, BORIS can selectively rescue expression of promoter-activated genes. 100
