iiinova — where your digital self keeps evolving

Replies

Mathias Munschauer @munschauerlab.bsky.social · 12/02/2024

Time to SHIFT to BlueSky and share our recent paper introducing SHIFTR, a novel platform for region-specific RNA interactome discovery developed by PhD student Jens Aydin 👨‍🔬 in my group. Now published in Nucleic Acid Research: academic.oup.com/nar/advance-...

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Mathias Munschauer @munschauerlab.bsky.social · 12/02/2024

With SHIFTR you can identify proteins directly bound to a specific region within any endogenous RNA. In addition to delivering region-resolution, SHIFTR requires orders of magnitude lower input material compared to the state-of-the-art, thus removing a critical bottleneck.

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Mathias Munschauer @munschauerlab.bsky.social · 12/02/2024

In brief, SHIFTR relies on the sequence-independent isolation of crosslinked RNA-protein complexes by organic phase separation as described in OOPS, XRNAX, or PTex.

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